Journal: International Journal of Molecular Sciences

Article Title: Cytokine-Modulated Natural Killer Cells Differentially Regulate the Activity of the Hepatitis C Virus

doi: 10.3390/ijms19092771

Figure Lengend Snippet: Effect of recombinant IL-21 on expression of CD56, STAT proteins, and IFN-γ in NK-92 cells. NK-92 cells were cultured in the presence or absence of recombinant IL-21 (0.2 ng/mL) for 6 h and stained with fluorescent anti-CD56 antibodies. ( A ) Representative FACS plots showing CD56 expression of NK-92; ( B ) % CD56 +dim NK-92. Alternatively, NK-92 cells were incubated with or without recombinant IL-21 (0.2 ng/mL) for 6 h and subsequently cocultured with J6/JFH-1-huh 7.5 cells or naïve huh 7.5 cells for 12 h. Cell lysates from NK-92 cells only were assessed for the expressions of STAT1 and STAT5 proteins. β-actin was served as the loading control. IFN-γ production (pg/mL) in culture supernatants was determined by ELISA. ( C ) Expressions of STAT1 and STAT5 proteins by Western blot; ( D ) Relative band intensity of STAT1 and STAT5 proteins compared with the loading control from at least three independent experiments; ( E ) IFN-γ production (pg/mL).

Article Snippet: Cell free supernatants were harvested to measure the production of IFN-γ using a human enzyme linked immunosorbent assay kit (BD Biosciences).

Techniques: Recombinant, Expressing, Cell Culture, Staining, Incubation, Enzyme-linked Immunosorbent Assay, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Tear Proteomics Study of Dry Eye Disease: Which Eye Do You Adopt as the Representative Eye for the Study?

doi: 10.3390/ijms22010422

Figure Lengend Snippet: Comparison of the expression of 2 representative biochemical markers in both eyes of dry eye patients ( N = 23). ( a ) concentration of tear matrix metallopeptidase 9 (MMP-9) ( p = 0.9881); ( b ) concentration of tear lactoferrin ( p > 0.9999); and ( c ) concentration ratio of MMP-9 to lactoferrin of each tear sample ( p = 0.7540). ( d ) correlation of tear MMP-9 concentration; ( e ) correlation of tear lactoferrin concentration; ( f ) correlation of concentration ratio of MMP-9 to lactoferrin of binocular tear samples of each patients. Abbreviation: LF, lactoferrin.

Article Snippet: The lactoferrin concentrations of all tear samples were measured using a human lactoferrin enzyme-linked immunosorbent assay (ELISA) kit (Catalog no. KA0484, Abnova, Taipei, Taiwan).

Techniques: Comparison, Expressing, Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: Tear Proteomics Study of Dry Eye Disease: Which Eye Do You Adopt as the Representative Eye for the Study?

doi: 10.3390/ijms22010422

Figure Lengend Snippet: Comparison of lactoferrin-corrected standardized signals for six always presented molecules in binocular tear samples of dry eye subjects ( N = 23). ( a ) lactoferrin ( p = 0.8422); ( b ) lipocalin-1 ( p = 0.6221); ( c ) lysozyme C ( p = 0.9168); ( d ) Immunoglobulin alpha-1 chain C region ( p = 0.4820); ( e ) lacritin ( p = 0.5803); and ( f ) prolactin-inducible protein ( p = 0.3604). Abbreviation: Ig, immunoglobulin.

Article Snippet: The lactoferrin concentrations of all tear samples were measured using a human lactoferrin enzyme-linked immunosorbent assay (ELISA) kit (Catalog no. KA0484, Abnova, Taipei, Taiwan).

Techniques: Comparison

Journal: International Journal of Molecular Sciences

Article Title: Tear Proteomics Study of Dry Eye Disease: Which Eye Do You Adopt as the Representative Eye for the Study?

doi: 10.3390/ijms22010422

Figure Lengend Snippet: Binocular association of lactoferrin-corrected standardized signals for six always presented molecules in tear samples of dry eye patients. ( a ) lactoferrin; ( b ) lipocalin-1; ( c ) lysozyme C ( d ) immunoglobulin alpha-1 chain C region; ( e ) lacritin; and ( f ) prolactin-inducible protein. Abbreviation: Ig, immunoglobulin.

Article Snippet: The lactoferrin concentrations of all tear samples were measured using a human lactoferrin enzyme-linked immunosorbent assay (ELISA) kit (Catalog no. KA0484, Abnova, Taipei, Taiwan).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Tear Proteomics Study of Dry Eye Disease: Which Eye Do You Adopt as the Representative Eye for the Study?

doi: 10.3390/ijms22010422

Figure Lengend Snippet: Association between representative ocular surface homeostatic markers and tear biomarkers in the ipsilateral eye. ( a ) right eye ( N = 23); ( b ) left eye ( N = 23). Abbreviation: OSDI, ocular surface disease index; TMH_c, central tear meniscus height; BR_t, temporal bulbar redness score; NIKBUT_f, non-invasive keratographic first break-up time; NIKBUT_at, assessable time of non-invasive keratographic break-up test; Oxford, Oxford staining score; MeS, meiboscale; LF, lactoferrin; IGHA1, immunoglobulin alpha-1 chain C region; and PIP, prolactin-inducible protein.

Article Snippet: The lactoferrin concentrations of all tear samples were measured using a human lactoferrin enzyme-linked immunosorbent assay (ELISA) kit (Catalog no. KA0484, Abnova, Taipei, Taiwan).

Techniques: Staining

Journal: International Journal of Molecular Sciences

Article Title: Tear Proteomics Study of Dry Eye Disease: Which Eye Do You Adopt as the Representative Eye for the Study?

doi: 10.3390/ijms22010422

Figure Lengend Snippet: Association between ocular surface homeostasis and tear biomarkers in the ipsilateral eye.

Article Snippet: The lactoferrin concentrations of all tear samples were measured using a human lactoferrin enzyme-linked immunosorbent assay (ELISA) kit (Catalog no. KA0484, Abnova, Taipei, Taiwan).

Techniques: